Molecular detection of bacteria in plant tissues, using universal 16S ribosomal DNA degenerated primers
نویسندگان
چکیده
منابع مشابه
Molecular detection of bacteria in plant tissues, using universal 16S ribosomal DNA degenerated primers
Highly specific, sensitive and rapid tests are required for the detection and identification of covert bacterial contaminations in plant tissue cultures. Current methods available for this purpose are tedious, time consuming, highly error prone, expensive, require advanced technical expertise and are sometimes ineffective. We report here the development of a sensitive polymerase chain reaction ...
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Background: There is a conserved portion in the 16S rRNA gene of bacteria which can be amplified by the universal PCR method. This fragment is 996 bp in length. In this method, only one set of universal primers is used for the amplification of the conserved region of the 16S rRNA gene, in common bacterial pathogens. Therefore, using the universal PCR method, these bacteria are detectable on...
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background: there is a conserved portion in the 16s rrna gene of bacteria which can be amplified by the universal pcr method. this fragment is 996 bp in length. in this method, only one set of universal primers is used for the amplification of the conserved region of the 16s rrna gene, in common bacterial pathogens. therefore, using the universal pcr method, these bacteria are detectable only b...
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ژورنال
عنوان ژورنال: Biotechnology & Biotechnological Equipment
سال: 2014
ISSN: 1310-2818,1314-3530
DOI: 10.1080/13102818.2014.937139